Among the most important techniques in proteome analyses is the in-gel digestion of protein spots/bands that have been resolved by electrophoresis using digestive enzymes, such as trypsin and lysyl endopoptidase®. Proteins can be identified by mass spectrometry analysis of the peptides produced by in-gel digestion, and further information regarding post-translational modifications can be obtained.
Lysyl Endopeptidase®, Mass Spectrometry Grade (Lys-C) is a freeze dried product that retained sufficient activity for in-gel digestion and packed in very small quantities for convenience purposes.