The appropriate method of total protein determination varies depending on the sample. While the bicinchoninic acid (BCA) assay is less sensitive to detergents and urea in the sample compared to some other methods, chelating agents such as EDTA inhibit the assay. The Bradford assay is less susceptible to inhibition by reducing agents or chelating agents in the sample, but it is inhibited by detergents. Pyrogallol red-molybdenum assay is used for protein determination in urine and cerebrospinal fluid samples. Fujifilm Wako offers protein determination reagents according to researcher's purpose.