FUJIFILM Wako Phenylisothiocyanate (PITC), as known as Edman's Reagent, is a high-purity reagent for pre-column quantitative derivatization of amino acids by HPLC. PITC reacts with the amino acid residue at the amino terminus under basic conditions to form a phenylthiocarbamyl derivative (PTC-protein). Our Wakopak® Wakosil® columns provide easy and reproducible amino acids separation from the amino acid derivative.