Thin-Layer Chromatography (TLC) is a chromatography using a thin layer plate prepared by fixing an adsorbent fixed in thin film.
When one end of the thin layer plate is immersed in a solvent, the solvent migrates upward through gaps (capillary phenomenon). When a sample chemical is present on the plate, the sample also moves in a way being dragged by the solvent migration. At this time, the distance (Rf value) of sample migration differs depending on the difference / balance between the degree of adsorption to the fixed layer (adsorbent) and the affinity to the mobile layer (solvent). This principle is utilized to separate and identify organic compounds.
Selection of TLC stain is also important to obtain good results in TLC analysis. For example, if you want to detect compounds with an amino group from multiple spots, you can selectively stain by using ninhydrin TS.
Common TLC stains, preparation method of stains, and stained compounds are shown below. FUJIFILM Wako offers ready-prepared product in addition to reagents as raw materials for stains. Utilize them according to the intended purposes.