Wakosil®-II 5SIL-AQ is a high-purity silica gel packing agent for HPLC.
Superior analysis of highly polar substances is possible, especially basic substances with low retention that are difficult to analyze with common ODS packing agents, further broadening the scope of HPLC analysis.
Wakopak® Combi ODS, Wakopak® Combi ODS fast
Wakopak® Combi ODS series adopts high-purity ODS (5 μm, particle size). They can be applied to a wide-range of compounds, including hydrophilic/hydrophobic compounds and metal-coordination compounds.
Wakopak® Combi ODS series are designed to minimize pressure loss to support gradient methods that enable separation in a short time. Wakopak® Combi ODS fast series uses a packing agent with a particle size of 3 μm to achieve further reduction of column length and analysis time.
| Column | ：Wakopak® Combi ODS fast (4.6 mm x 30 mm) | Wakopak® Combi ODS (4.6 mm x 50 mm) |
| Flow Rate | ：3.0 mL/min. | 3.0 mL/min. |
| Gradient | ：0 - 2.5 min. B 0 - 80% 2.5 - 3 min. B 80% | 0 - 3.5 min. B 0 - 80% 3.5 - 4.5 min. B 80% |
| Eluent | ：A) CH3CN/H2O = 5/59(v/v), 0.05% TFA B) CH3CN/H2O = 95/5(v/v), 0.05% TFA | |
| Temp | ：R.T. | |
| Detection | ：UV 280 nm 2.048AUFS | |
| Sample | ：1) p-Hydroxybenzoic Acid 2) Methyl p-hydroxybenzoate 3) Salicylic acid 4) Ethyl p-hydroxybenzoate 5) Propyl p-hydroxybenzoate 6) Methyl salicylate | 7) Butyl p-hydroxybenzoate 8) Ethyl salicylate 9) Propyl salicylate 10) Butyl salicylate each 1 mg/mL |
| Injection Volume | ：5 µL | |
Wakopak® Combi CN
Wakopak® Combi CN series adopts packing agent made by introducing the cyanopropyl group to high-purity silica gel with a particle size of 5 μm, followed by end capping. Compared with ODS (C18) column, the hydrophilic compounds feature high retention, whereas the hydrophobic compounds feature low retention.
| Column | ：Wakopak® Combi CN (4.6 mm x 50 mm) | Wakopak® Combi ODS (4.6 mm x 50 mm) |
| Flow Rate | ：2.0 mL/min. | 2.0 mL/min. |
| Eluent | ：A) CH3CN/H2O = 5/59(v/v), 0.05% TFA B) CH3CN/H2O = 95/5(v/v), 0.05% TFA 0 - 5.0 min. B 0 - 50% 5.0 min. B 50% | |
| Temp | ：R.T. | |
| Detection | ：UV 254 nm 2.048AUFS | |
| Sample | ：1) p-Hydroxybenzoic Acid 2) Methyl p-hydroxybenzoate 3) Ethyl p-hydroxybenzoate | 4) Propyl p-hydroxybenzoate 5) Butyl p-hydroxybenzoate each 0.2 mg/mL |