LabAssay™ Albumin
| Grade : | for Cellbiology |
| Storage Condition : | Keep at 2-10 degrees C. |
| Catalogue/Product Number | Package Size | COA Download |
|---|---|---|
|
291-98901
|
250Tests |
Document
Overview
Kit component
For 250 tests
| Chromogen Reagent | 65 mL/1 bottle |
|---|---|
| Bovine Albumin Standard | 1 bottle |
| Mouse Albumin Standard | 1 bottle |
| Rat Albumin Standard | 1 bottle |
Product Overview
Albumin is a plasma protein synthesized in the liver. Serum or plasma albumin levels are important indicators in the diagnosis and management of liver disease and hepatic dysfunction. In addition, kidney diseases characterized by protein leakage into the urine are known to cause decreases in blood total protein and albumin levels, leading to hypoalbuminemia.
By measuring both albumin and total protein concentrations, the albumin-to-globulin ratio (A/G ratio) can be determined. The A/G ratio serves as a valuable marker for a range of conditions, including liver disease, kidney disease, nutritional disorders, multiple myeloma, and chronic inflammatory conditions.
LabAssay™ Albumin, a biochemical assay kit based on the bromocresol green (BCG) method, providing a simple and rapid solution for measuring albumin in blood.
[Note] LabAssay™ series are reagents for research purposes. They cannot be used for diagnostic purposes.
Kit Performance
| Analysis sample *1 | Human Serum/Plasma (EDTA, heparin) Mouse Serum/Plasma (EDTA, heparin) Rat Serum/Plasma (EDTA, heparin) Dog Serum/Plasma (EDTA, heparin) Cat Serum/Plasma (EDTA, heparin) Culture medium (D-MEM, RPMI)*2 |
|---|---|
| Calibration curve range | 0.625-7.5 g/dL |
| Sample volume | 2 μL |
| Measurement duration | Approx. 20 min. |
| Wavelength | 630 nm |
*1 For animal species other than mice and rats, please use bovine albumin standards.
*2 It is tested by using the samples that standard substances have been added to the culture medium.
Measurement availability depends on the culture medium, cell type and culture conditions. Please perform a spiked recovery test or dilution linearity test in advance using the culture medium to be used in your experiment.
Example of Calibration Curve
Bovine Albumin Standard
Mouse Albumin Standard
Rat Albumin Standard
Assay Principle
When the Chromogen Reagent is added to a sample, albumin binds to bromocresol green (BCG) to form a blue-colored complex. Albumin concentration is determined by measuring the intensity of the blue color.
Data
Repeatability (within run precision)
Repeatability was conducted by measuring five-fold assays of serum/plasma (EDTA) samples from human at two concentrations with this product.
| n＼ID | Human serum | Human plasma (EDTA) | ||
|---|---|---|---|---|
| ID1 (g/dL) | ID2 (g/dL) | ID3 (g/dL) | ID4 (g/dL) | |
| 1 | 4.74 | 6.07 | 4.36 | 6.25 |
| 2 | 4.84 | 6.30 | 4.52 | 6.46 |
| 3 | 4.78 | 5.66 | 4.47 | 6.30 |
| 4 | 4.79 | 6.09 | 4.54 | 6.26 |
| 5 | 4.93 | 5.93 | 4.47 | 6.18 |
| mean | 4.82 | 6.01 | 4.47 | 6.29 |
| SD | 0.0730 | 0.236 | 0.0698 | 0.104 |
| CV(%) | 1.5 | 3.9 | 1.6 | 1.7 |
[Note] Sample ID-2 and 4 was spiked with a standard at a concentration of 2 g/dL.
[Result]
The CV (%) of human serum was 1.5-3.9% and human plasma (EDTA) was 1.6-1.7%, indicating good repeatability.
Reproducibility (between run precision)
Reproducibility was conducted by measuring serum/plasma (EDTA) samples from human at two concentrations each for four days with this product.
| Day＼ID | Human serum | Human plasma (EDTA) | ||
|---|---|---|---|---|
| ID9 (g/dL) | ID10 (g/dL) | ID11 (g/dL) | ID12 (g/dL) | |
| 1 | 6.99 | 4.44 | 6.33 | 3.75 |
| 2 | 7.05 | 4.71 | 6.47 | 3.90 |
| 3 | 6.52 | 4.32 | 7.10 | 3.84 |
| 4 | 6.46 | 4.44 | 6.93 | 3.51 |
| mean | 6.76 | 4.48 | 6.71 | 3.75 |
| SD | 0.308 | 0.165 | 0.366 | 0.171 |
| CV(%) | 4.6 | 3.7 | 5.5 | 4.6 |
[Result]
The CV (%) of human serum was 3.7-4.6% and human plasma (EDTA) was 4.6-5.5%, indicating good reproducibility.
Dilution Linearity Test
Serum and plasma (EDTA) from human, mouse, rat, dog and cat as well as culture medium (D-MEM and RPMI) spiked with the standard, were serially diluted twofold with buffer in this kit and measured in duplicate to assess linearity.
[Note] Sample ID-6 was spiked with standard at a concentration of 2 g/dL
[Note] Sample ID-8 was spiked with standard at a concentration of 2 g/dL
[Note] Sample ID-1 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-3 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-5 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-7 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-9 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-11 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-13 was spiked with standard at a concentration of 5 g/dL
[Note] Sample ID-15 was spiked with standard at a concentration of 5 g/dL
[Result]
All measurement results showed good linearity within the measurement range.
Spike and Recovery Test
Standard solutions at three concentrations were added to serum/plasma (EDTA) from human, mouse, rat, dog and cat as well as culture medium (D-MEM and RPMI). Next, spike recovery tests were conducted, with each measurement performed in duplicate.
Human sample
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | － | 4.99 | － | - |
| 1.39 | 6.29 | 1.30 | 93.2 | |
| 2.77 | 7.77 | 2.78 | 100 | |
| 5.54 | 10.3 | 5.31 | 95.8 | |
| Average | 96.5 | |||
| Plasma (EDTA) | － | 4.80 | － | - |
| 1.85 | 6.48 | 1.68 | 90.8 | |
| 2.77 | 7.48 | 2.68 | 96.8 | |
| 5.54 | 9.91 | 5.11 | 92.2 | |
| Average | 93.3 | |||
Mouse sample (BALB/c)
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | - | 0.72 | - | - |
| 1.40 | 2.25 | 1.53 | 109 | |
| 2.81 | 3.76 | 3.04 | 108 | |
| 5.62 | 6.42 | 5.70 | 101 | |
| Average | 106 | |||
| Plasma (EDTA) | - | 0.69 | - | - |
| 1.40 | 2.12 | 1.43 | 102 | |
| 2.81 | 3.68 | 2.99 | 106 | |
| 5.62 | 6.52 | 5.81 | 103 | |
| Average | 104 | |||
Rat sample (S.D. rat)
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | - | 3.12 | - | - |
| 1.40 | 4.40 | 1.28 | 91.4 | |
| 2.81 | 5.77 | 2.65 | 94.3 | |
| 5.62 | 8.54 | 5.42 | 96.4 | |
| Average | 94.1 | |||
| Plasma (EDTA) | - | 2.92 | - | - |
| 1.44 | 4.25 | 1.33 | 92.4 | |
| 2.87 | 5.51 | 2.59 | 90.2 | |
| 5.74 | 8.98 | 6.06 | 106 | |
| Average | 96.1 | |||
Dog sample (Beagle)
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | － | 2.39 | － | - |
| 1.90 | 4.18 | 1.79 | 94.2 | |
| 2.85 | 5.07 | 2.68 | 94.0 | |
| 5.69 | 7.93 | 5.54 | 97.4 | |
| Average | 95.2 | |||
| Plasma (EDTA) | － | 2.24 | － | - |
| 1.89 | 3.95 | 1.71 | 90.5 | |
| 2.84 | 4.85 | 2.61 | 91.9 | |
| 5.68 | 8.22 | 5.98 | 105 | |
| Average | 95.9 | |||
Cat sample (Japanese cat)
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| Serum | － | 3.41 | － | - |
| 1.67 | 4.95 | 1.54 | 92.2 | |
| 2.50 | 5.73 | 2.32 | 92.8 | |
| 5.00 | 8.20 | 4.79 | 95.8 | |
| Average | 93.6 | |||
| Plasma (EDTA) | － | 2.31 | － | - |
| 1.75 | 4.13 | 1.82 | 104 | |
| 2.63 | 4.77 | 2.46 | 93.5 | |
| 5.26 | 7.44 | 5.13 | 97.5 | |
| Average | 98.4 | |||
Culture medium
| Spiked amount (g/dL) | Measurement value (g/dL) | Recovery volume (g/dL) | Recovery rate (%) | |
|---|---|---|---|---|
| D-MEM | - | 0.00 | - | - |
| 1.18 | 1.14 | 1.14 | 96.6 | |
| 2.96 | 2.97 | 2.97 | 100 | |
| 5.92 | 6.39 | 6.39 | 108 | |
| Average | 102 | |||
| RPMI | - | 0.00 | - | - |
| 1.23 | 1.13 | 1.13 | 91.9 | |
| 3.07 | 2.94 | 2.94 | 95.8 | |
| 6.15 | 6.71 | 6.71 | 109 | |
| Average | 98.9 | |||
[Result]
Good recovery rates were confirmed.
Correlation with Automated Analyzer Reagents
Albumin levels in serum/plasma (EDTA) samples from human were measured using LabAssay™ Albumin and reagents for automated analyzers, and the correlation between the two sets of results was evaluated.
[Result]
A strong correlation was confirmed between the values obtained with LabAssay™ Albumin and those obtained using reagents for automated analyzers.
Measurement Using Actual Samples
Human sample
| ID＼n | Measurement value (g/dL) n=2 (Average) | ||
|---|---|---|---|
| Serum | ID1 | 5.17 | |
| ID2 | 5.11 | ||
| ID3 | 5.80 | ||
| Plasma (EDTA) | ID1 | 4.50 | |
| ID2 | 5.52 | ||
| ID3 | 5.61 | ||
Mouse sample (BALB/c)
| ID＼n | Measurement value (g/dL) | mean | SD | CV (%) | ||
|---|---|---|---|---|---|---|
| 1 | 2 | |||||
| Serum | ID1 | 3.42 | 3.39 | 3.41 | 0.0212 | 0.6 |
| ID2 | 3.93 | 3.83 | 3.88 | 0.0707 | 1.8 | |
| ID3 | 2.68 | 2.69 | 2.69 | 0.00707 | 0.3 | |
| Plasma (EDTA) | ID1 | 1.87 | 1.88 | 1.88 | 0.00707 | 0.4 |
| ID2 | 2.40 | 2.27 | 2.34 | 0.0919 | 3.9 | |
| ID3 | 1.99 | 1.90 | 1.95 | 0.0636 | 3.3 | |
Rat sample (S.D. rat)
| ID＼n | Measurement value (g/dL) | mean | SD | CV (%) | ||
|---|---|---|---|---|---|---|
| 1 | 2 | |||||
| Serum | ID1 | 3.99 | 3.95 | 3.97 | 0.0283 | 0.7 |
| ID2 | 3.83 | 3.75 | 3.79 | 0.0566 | 1.5 | |
| ID3 | 3.59 | 3.58 | 3.59 | 0.00707 | 0.2 | |
| Plasma (EDTA) | ID1 | 3.58 | 3.50 | 3.54 | 0.0566 | 1.6 |
| ID2 | 3.43 | 3.55 | 3.49 | 0.0849 | 2.4 | |
| ID3 | 3.36 | 3.42 | 3.39 | 0.0424 | 1.3 | |
Dog sample (Beagle)
| ID＼n | Measurement value (g/dL) | mean | SD | CV (%) | ||
|---|---|---|---|---|---|---|
| 1 | 2 | |||||
| Serum | ID1 | 3.46 | 3.46 | 3.46 | 0.00 | 0.0 |
| ID2 | 2.63 | 2.65 | 2.64 | 0.0141 | 0.5 | |
| ID3 | 3.02 | 3.20 | 3.11 | 0.127 | 4.1 | |
| Plasma (EDTA) | ID1 | 2.81 | 2.81 | 2.81 | 0.00 | 0.0 |
| ID2 | 2.56 | 2.61 | 2.59 | 0.0354 | 1.4 | |
| ID3 | 2.78 | 2.78 | 2.78 | 0.00 | 0.0 | |
Cat sample (Japanese cat)
| ID＼n | Measurement value (g/dL) | mean | SD | CV (%) | ||
|---|---|---|---|---|---|---|
| 1 | 2 | |||||
| Serum | ID1 | 2.72 | 2.72 | 2.72 | 0.00 | 0.0 |
| ID2 | 3.27 | 2.98 | 3.13 | 0.205 | 6.6 | |
| ID3 | 3.07 | 3.08 | 3.08 | 0.00707 | 0.2 | |
| Plasma (EDTA) | ID1 | 3.03 | 3.04 | 3.04 | 0.00707 | 0.2 |
| ID2 | 2.21 | 2.19 | 2.20 | 0.0141 | 0.6 | |
| ID3 | 2.39 | 2.41 | 2.40 | 0.0141 | 0.6 | |
Example Calculation of the Albumin-to-Globulin Ratio (A/G Ratio)
The blood A/G ratio can be determined by using LabAssay™ Albumin (this product) in conjunction with LabAssay™ Total Protein (Product No. 295-98801). The A/G ratio expresses the proportion of albumin to globulin, where globulin is defined as total protein minus albumin. The formula is shown below, along with representative calculations for human, mouse, rat, dog, and cat samples.
Human sample
| Sample | No. | Total protein* (g/dL) | Albumin (g/dL) | A/G ratio |
|---|---|---|---|---|
| Serum | 1 | 8.50 | 5.17 | 1.6 |
| 2 | 7.43 | 5.11 | 2.2 | |
| 3 | 9.74 | 5.80 | 1.5 | |
| Plasma (EDTA) | 4 | 7.48 | 4.50 | 1.5 |
| 5 | 8.52 | 5.52 | 1.8 | |
| 6 | 8.93 | 5.61 | 1.7 |
Mouse sample (BALB/c)
| Sample | No. | Total protein* (g/dL) | Albumin (g/dL) | A/G ratio |
|---|---|---|---|---|
| Serum | 1 | 4.98 | 3.40 | 2.2 |
| 2 | 5.49 | 3.88 | 2.4 | |
| 3 | 5.23 | 2.68 | 1.1 | |
| Plasma (EDTA) | 4 | 2.96 | 1.88 | 1.7 |
| 5 | 4.81 | 2.34 | 1.0 | |
| 6 | 3.13 | 1.95 | 1.6 |
Rat sample (S.D. rat)
| Sample | No. | Total protein* (g/dL) | Albumin (g/dL) | A/G ratio |
|---|---|---|---|---|
| Serum | 1 | 7.91 | 3.97 | 1.0 |
| 2 | 7.35 | 3.79 | 1.1 | |
| 3 | 7.61 | 3.59 | 0.9 | |
| Plasma (EDTA) | 4 | 6.44 | 3.54 | 1.2 |
| 5 | 6.51 | 3.49 | 1.2 | |
| 6 | 6.28 | 3.39 | 1.2 |
Dog sample (Beagle)
| Sample | No. | Total protein* (g/dL) | Albumin (g/dL) | A/G ratio |
|---|---|---|---|---|
| Serum | 1 | 6.31 | 3.46 | 1.2 |
| 2 | 4.51 | 2.64 | 1.4 | |
| 3 | 5.78 | 3.11 | 1.2 | |
| Plasma (EDTA) | 4 | 4.73 | 2.81 | 1.5 |
| 5 | 5.01 | 2.59 | 1.1 | |
| 6 | 7.07 | 2.78 | 0.7 |
Cat sample (Japanese cat)
| Sample | No. | Total protein* (g/dL) | Albumin (g/dL) | A/G ratio |
|---|---|---|---|---|
| Serum | 1 | 5.45 | 2.72 | 1.0 |
| 2 | 6.20 | 3.13 | 1.0 | |
| 3 | 6.54 | 3.08 | 0.9 | |
| Plasma (EDTA) | 4 | 5.96 | 3.03 | 1.0 |
| 5 | 5.08 | 2.20 | 0.8 | |
| 6 | 4.41 | 2.40 | 1.2 |
- Total protein was measured using our LabAssay™ Total Protein (Product No. 295-98801).